Anezaki Mamori – A childhood friend of Sena that has been protecting him from bullies since primary school. She is very protective of Sena. She is oblivious to the fact that Sena is Eyeshield 21 and thinks that Sena is just the football clubs manager. She joins the football club as another manager to protect Sena from Hiruma. She eventually becomes an excellent manager who knows the rules, analyzes the enemies strategies and make strategies for her team. She also keeps the club room very clean. Mamori begins to appreciate Hiruma later on.
The Ha-ha brothers(Kuroki Koji, Jumongi Kazuki, Togano Shozo) – The three bullies who chase Sena in his first day in Deimon. They join the team as linemen after Hiruma blackmails them, despite being forced to join they start to like football specially when they learned that Eyeshield 21 is actually Sena. They resolved that if Sena can change then they can too. They start out as complete noobs but quickly become very capable linemen due to the fact that they are always brawling in the streets.
Manabu Yukimitsu – A second year student who has been forced by his mother to dedicate his whole life to studies. He questions his way of life and looks back and he sees that he has not a one happy memory. He decides to join the football team after seeing Eyeshield play. He admires Eyeshields bravery the most. Yukimitsu enters the try outs but despite him failing Hiruma still accepts him as an act of kindness and seeing how hard he worked. He isn’t able to play until later though.
Daikichi Komusubi – Is one of the linemen of the Daimon Devil Bats. He is highly impressed of Kurita’s strength and decides to become his apprentice. He was able to join after passing the tryout along the Ha-ha brothers and Yukimitsu. He doesn’t talk much and speaks only through strange grunts and one word sentences.
Suzuna Taki – A blue haired girl who always wears rollerblades. Working as a reporter hired to discover Eyeshields true identity. She becomes the teams cheerleader and develops a relationship with Sena later on.
Natsuhiko Taki – A high school student who has a dream of becoming an NFL player. He is foolishly overconfident and has the odd habit of flinging his shirt open and spinning around like a ballerina. Is usually called an idiot by other people including her Sister Suzuna. He encounters Sena during a tryout in the United States but after failing to qualify Sena asks him to join the Deimon Devil bats. He possesses great flexibility and good all around skills which made him ideal for the “tight end” position.
Doburoku Sakaki – Is the coach of the Deimon Devil Bats and a former player himself. He trains Hiruma, Musashi and Kurita before they join the highschool. However after some unpayable debts because of his gambling habits he flees Japan for the United States. He returns to Japan after Hiruma pays all his debts.He and Ojo’s trainer and coach, Gunpei Shoji, were teammates at Sengoku University’s American football team, and were known as “The Twin Japanese Blades”.
Gen “Musashi” Takekura – Alias the 60 yard magnum, often mistaken as a much older person. One of the three founding members of the Devil Bats along with Hiruma and Kurita. He first met Kurita on a job from his father to fix a hole in the American base where Hiruma sneaks in to. After listening to Kurita’s pleas he still fixes the hole but then kicks another one. He later found out that they go to the same middle school and then forms the Mao Devil Bats. He leaves the team on his freshman year because his father became ill and he had to take over with the family business. He was forced to drop out of school. Although he really wanted to keep playing he rejoins the Deimon Devil Bats much later after.
Tetsuo Ishimaru – Is the Devil Bats 2nd runningback. Recruited from the track team by Sena after helping him deliver newspapers. Ishimaru is considered an extremely normal person and therefore no one feels his presence in the field. Nevertheless Ishimaru’s obscurity works to his advantage when playing since no one notices when he is handed the ball.